circRNA basic information
circBase ID: -
Name: hsa_circ_PRKCI
Synonym: circ-PRKCI
Host Gene: PRKCI
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

HCCLM3; THLE-3
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UN
Associated gene: FOXK1, HK2, GLUT1, LDHA
Associated microRNA: miR-1294, miR-186-5p
Biological function: promotes viability, invasion and migration of HCC cells; promotes glycolysis
Molecular mechanism: ceRNA sponge of miR-1294 and miR-186-5p to upregulate FOXK1, thereby increasing glycolysis-related proteins (HK2, GLUT1, LDHA) and glucose/lactic acid levels
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis
Clinical significance:

-
Description:

In HCCLM3 hepatocellular carcinoma cells, circ-PRKCI acts as a ceRNA that sponges miR-1294 and miR-186-5p, resulting in increased FOXK1 expression. This enhances malignant phenotypes (viability, migration, invasion) and promotes glycolysis, including upregulation of HK2/GLUT1/LDHA and increased glucose/lactic acid levels.
Confidence score:

0.5607
Other information
Title:

circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma.

Journal: Molecular medicine reports
Published: 2021
PubMed ID: 33880589
Study type:

biological research
Data availability: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -