| Expression pattern: |
UP |
| Associated gene: |
TXNIP |
| Associated microRNA: |
miR-128-3p |
| Biological function: |
promotes viability and angiogenesis of retinal vascular endothelial cells and impairs blood-retinal barrier; circFTO silencing inhibits angiogenesis and promotes BRB recovery in vivo |
| Molecular mechanism: |
ceRNA mechanism: circFTO sponges miR-128-3p to upregulate TXNIP |
| Biological pathway or process: |
angiogenesis (promotes); proliferation (promotes); blood-retinal barrier breakdown (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Back-Splice Junction PCR / divergent primers PCR; RT-qPCR; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Tube Formation Assay; Western Blot; In Vivo Animal Model; H&E Staining |
| Clinical significance: |
- |
| Description: |
circFTO is up-regulated in high-glucose retinal vascular endothelial cells and in diabetic retinopathy mouse retinas. It promotes endothelial cell viability/angiogenesis and blood-retinal barrier breakdown by sponging miR-128-3p to increase TXNIP expression. |
| Confidence score: |
0.7537 |