| Expression pattern: |
UP |
| Associated gene: |
TJP1, FBXW4 mRNA |
| Associated microRNA: |
miR-324-3p |
| Biological function: |
Regulates trophoblast cell proliferation, invasion, and apoptosis; knockdown promoted HTR-8/SVneo cell proliferation and invasion and inhibited apoptosis, whereas overexpression reduced proliferation and invasion and promoted apoptosis. |
| Molecular mechanism: |
circFBXW4 acts as a miRNA sponge for miR-324-3p and regulates TJP1 expression through the circFBXW4/miR-324-3p/TJP1 axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); invasion (inhibits); apoptosis (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Clinical Sample Validation; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; TUNEL |
| Clinical significance: |
May be a promising therapeutic target and provide a novel perspective for early diagnosis and potential treatment of RSA. |
| Description: |
circFBXW4 is significantly up-regulated in placental villi from RSA patients. It acts through a circFBXW4/miR-324-3p/TJP1 ceRNA axis and regulates trophoblast proliferation, invasion, and apoptosis, suggesting potential relevance to RSA pathogenesis and therapy. |
| Confidence score: |
0.8035 |