| Expression pattern: |
UP |
| Associated gene: |
HMGB1, AGO2 |
| Associated microRNA: |
miR-1179 |
| Biological function: |
promotes PTC cell proliferation and tumor growth; facilitates cell-cycle progression |
| Molecular mechanism: |
acts as a miRNA sponge to sequester miR-1179, thereby upregulating HMGB1 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
Elevated expression of circFOXM1 was positively correlated with tumor size, tumor stage, and poor lymph node metastasis. |
| Description: |
circFOXM1 (hsa_circ_0025033), derived from FOXM1, is up-regulated in papillary thyroid carcinoma tissues and cell lines and is mainly localized in the cytoplasm. It promotes PTC proliferation and in vivo tumor growth by acting as a ceRNA sponge for miR-1179, thereby increasing HMGB1 expression. |
| Confidence score: |
0.8397 |