circRNA basic information
circBase ID: hsa_circ_0006732
Name: hsa_circ_ZDHHC20
Synonym: circZDHHC20
Host Gene: ZDHHC20
Genomic location(hg19): chr13:21987790-21999817:-
Genomic location(hg38): chr13:21413651-21425678:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; matched paracancerous normal intestinal tissues; CRC tumor tissues; normal tissues
Cell lines:

T84; LoVo; SW480; HCT116; HCT8; NCM460
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: RAB3D / Ras-related protein Rab-3D (Rab3D)
Associated microRNA: miR-127-5p
Biological function: Promotes CRC cell proliferation, migration, invasion and EMT; hsa_circ_0006732 knockdown suppresses these malignant biological activities.
Molecular mechanism: hsa_circ_0006732 functions as a ceRNA by sponging miR-127-5p to regulate RAB3D expression, thereby affecting proliferation, migration, invasion and EMT in CRC cells.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

hsa_circ_0006732 expression was associated with CRC clinicopathologic features including tumor size and TNM stage, indicated poor prognosis of CRC patients, and might serve as a potential therapeutic target.
Description:

hsa_circ_0006732 is up-regulated in colorectal cancer tissues and cell lines and is associated with tumor size, TNM stage and poor prognosis. Functionally, it promotes CRC cell proliferation, migration, invasion and EMT. Mechanistically, it acts as a ceRNA that sponges miR-127-5p to regulate RAB3D.
Confidence score:

0.6521
Other information
Title:

Hsa_circ_0006732 regulates colorectal cancer cell proliferation, invasion and EMT by miR-127-5p/RAB3D axis.

Journal: Molecular and cellular biochemistry
Published: 2022
PubMed ID: 35616807
Study type:

combined biological and clinical study
Data availability: All data generated or analyzed during this study are included in this published article.
Code availability: -