| Expression pattern: |
UP |
| Associated gene: |
PRMT7 |
| Associated microRNA: |
miR-1178 |
| Biological function: |
circ_0039960 promotes BC cell viability, cell cycle progression and Warburg effect, and inhibits apoptosis; silencing circ_0039960 inhibits cell growth and Warburg effect and induces cell cycle arrest and apoptosis. |
| Molecular mechanism: |
circ_0039960 acts as a sponge of miR-1178 to positively regulate PRMT7 expression, thereby regulating BC cell growth and Warburg effect. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; ELISA; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0039960 expression was associated with poor survival rate in BC patients and the findings may provide evidence for BC diagnostic and therapeutic targets. |
| Description: |
circ_0039960 is up-regulated in breast cancer tissues and cell lines, and high expression is associated with poor survival. Functionally, circ_0039960 promotes BC cell growth, cell cycle progression and Warburg effect while suppressing apoptosis. Mechanistically, circ_0039960 sponges miR-1178 to positively regulate PRMT7 expression, forming a circ_0039960/miR-1178/PRMT7 axis. |
| Confidence score: |
0.87 |