| Expression pattern: |
DN |
| Associated gene: |
PDCD4 |
| Associated microRNA: |
miR-1323 |
| Biological function: |
Restrains DDP resistance, proliferation, migration, invasion and glycolytic metabolism; promotes apoptosis; enhances DDP sensitivity and antitumor effect in xenograft tumors. |
| Molecular mechanism: |
Circ_0030998 acts as a miR-1323 sponge and positively regulates PDCD4 expression, thereby suppressing DDP resistance and NSCLC progression through the miR-1323/PDCD4 axis. |
| Biological pathway or process: |
chemoresistance (inhibits); drug resistance (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); glycolysis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ_0030998 expression was negatively correlated with TNM stage and was reduced in DDP-resistant NSCLC specimens, suggesting potential relevance to disease progression and chemoresistance. |
| Description: |
Circ_0030998 is a LAMP1-derived circRNA down-regulated in NSCLC, especially in DDP-resistant NSCLC tissues and cell lines. It suppresses cisplatin resistance and malignant phenotypes by sponging miR-1323 and up-regulating PDCD4, thereby inhibiting proliferation, migration, invasion and glycolysis while promoting apoptosis. Its reduced expression correlates with higher TNM stage and chemoresistance, suggesting potential therapeutic relevance. |
| Confidence score: |
0.8443 |