| Expression pattern: |
UP |
| Associated gene: |
HMGA2, EIF4A3, ZCCHC7 mRNA |
| Associated microRNA: |
miR-618 |
| Biological function: |
circ_0001860 promotes gastric cancer cell growth, proliferation, invasion, immune evasion, and xenograft tumor growth; knockdown of circ_0001860 suppresses these malignant phenotypes. |
| Molecular mechanism: |
EIF4A3 positively regulates circ_0001860 expression by binding to ZCCHC7 mRNA; circ_0001860 acts as a ceRNA by sponging miR-618 to positively modulate HMGA2 expression, thereby promoting gastric cancer progression and immune evasion. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Transwell Assay; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0001860 upregulation indicates poor prognosis, shorter overall survival, more lymph node metastasis, and higher TNM stage in gastric cancer patients; it may offer a potential target for clinical detection and treatment. |
| Description: |
circ_0001860 is upregulated in gastric cancer tissues and cell lines and is associated with shorter overall survival and more aggressive clinicopathological features. EIF4A3 induces circ_0001860 expression, and circ_0001860 promotes gastric cancer growth, invasion, and immune evasion by sponging miR-618 to increase HMGA2 expression. Silencing circ_0001860 suppresses malignant phenotypes in vitro and reduces xenograft tumor growth in vivo. |
| Confidence score: |
0.8066 |