| Expression pattern: |
UP |
| Associated gene: |
IGF1R, PI3K, AKT, mTOR, Ago2 |
| Associated microRNA: |
miR-143-3p |
| Biological function: |
Promotes gastric cancer cell proliferation, growth, migration and invasion; acts as a tumor-promoter in gastric cancer. |
| Molecular mechanism: |
ceRNA mechanism: circ_0006089 sponges miR-143-3p, increases IGF1R expression, and activates the PI3K/Akt/mTOR signaling pathway. |
| Biological pathway or process: |
PI3K/AKT/mTOR (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; BrdU; Wound Healing Assay; Transwell Assay; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0006089 expression was associated with shorter overall survival, higher TNM stage, larger tumor size and positive lymph node metastasis in GC patients; circ_0006089 may serve as a promising therapeutic target for GC. |
| Description: |
circ_0006089 is up-regulated in gastric cancer tissues and cell lines, and high expression is associated with poorer overall survival and more aggressive clinicopathological features. Functionally, circ_0006089 promotes GC cell proliferation, migration and invasion. Mechanistically, it acts as a ceRNA for miR-143-3p, thereby increasing IGF1R expression and activating the PI3K/Akt/mTOR pathway. |
| Confidence score: |
0.7855 |