| Expression pattern: |
DN |
| Associated gene: |
NF90, TJP1 mRNA |
| Associated microRNA: |
- |
| Biological function: |
circCCNB1 inhibits NPC cell migration and invasion, enhances tight junctions between cancer cells, and functions as a tumor suppressor in NPC migration and invasion. |
| Molecular mechanism: |
circCCNB1 directly binds TJP1 mRNA and interacts with the RNA-binding protein NF90, promoting NF90 binding to TJP1 mRNA, stabilizing TJP1 mRNA, and increasing TJP1 expression. |
| Biological pathway or process: |
migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; IF (Immunofluorescence); Clinical Sample Validation; RIP (RNA Immunoprecipitation); circRIP; RNA Pull-Down; Transfection; MTT; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circCCNB1 may provide potential markers and targets for the diagnosis and treatment of patients with NPC. |
| Description: |
circCCNB1 is downregulated in NPC tissues and cell lines and suppresses NPC cell migration and invasion. Mechanistically, it binds TJP1 mRNA and NF90, enhances NF90-TJP1 mRNA interaction, stabilizes TJP1 mRNA, and increases TJP1 expression, thereby strengthening tight junction-related suppression of metastasis-related phenotypes. |
| Confidence score: |
0.8478 |