| Expression pattern: |
UP |
| Associated gene: |
REL, NF-kappaB pathway, NF-kappaB p65, p-NF-kappaB p65 |
| Associated microRNA: |
miR-1263 |
| Biological function: |
Promotes proliferation, cell cycle progression and inflammatory response, inhibits apoptosis, and facilitates RA progression in HFLS-RA cells. |
| Molecular mechanism: |
circ_0088036 acts as a sponge for miR-1263, increases REL expression, and activates the NF-kappaB signaling pathway. |
| Biological pathway or process: |
NF-kappaB (promotes); proliferation (promotes); cell cycle (promotes); inflammation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_0088036 might be a useful biomolecule in the diagnosis of RA. |
| Description: |
circ_0088036 is up-regulated in sera and HFLS-RA cells from RA patients. It promotes HFLS-RA proliferation, cell cycle progression and inflammatory cytokine production while suppressing apoptosis, mainly through sponging miR-1263 to increase REL and activate NF-kappaB signaling. The study also suggests that its abnormal up-regulation may have diagnostic relevance in RA. |
| Confidence score: |
0.7982 |