| Expression pattern: |
UP |
| Associated gene: |
SSR1, PBX2, AGO2, JAK1, STAT1, STAT3 |
| Associated microRNA: |
miR-452-5p |
| Biological function: |
promotes glioma cell proliferation, migration, invasion and tumor growth in vivo |
| Molecular mechanism: |
PBX2-mediated circTLK1 acts as a ceRNA to sponge miR-452-5p, increasing SSR1 and activating JAK/STAT signaling |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
provides new insights into glioma diagnosis and therapy |
| Description: |
circTLK1 is up-regulated in glioma tissues and cell lines and promotes glioma cell proliferation, migration, invasion, and xenograft growth. Mechanistically, PBX2 enhances circTLK1 expression; circTLK1 functions as a ceRNA sponging miR-452-5p to upregulate SSR1 and activate JAK/STAT signaling. |
| Confidence score: |
0.8586 |