| Expression pattern: |
UP |
| Associated gene: |
FZD4, Ago2, SUSD1 |
| Associated microRNA: |
miR-326 |
| Biological function: |
accelerates proliferation and inflammation and inhibits apoptosis of TNF-a-treated RA-FLSs; promotes cell cycle progression; contributes to RA progression |
| Molecular mechanism: |
circ_0088036 acts as a miR-326 sponge and up-regulates FZD4 expression through the circ_0088036/miR-326/FZD4 axis |
| Biological pathway or process: |
proliferation (promotes); inflammation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0088036 expression was positively correlated with disease activity marker DAS28-ESR in RA patients and may be implicated in RA progression |
| Description: |
circ_0088036 is up-regulated in synovial tissues from RA patients and in TNF-a-treated RA-FLSs. It promotes RA-FLS proliferation, cell cycle progression and inflammatory cytokine production while inhibiting apoptosis, partly by sponging miR-326 to increase FZD4 expression. Its expression correlates positively with DAS28-ESR, suggesting relevance to RA disease activity. |
| Confidence score: |
0.7795 |