circRNA basic information
circBase ID: hsa_circ_0000520
Name: hsa_circ_RPPH1
Synonym: circ_0000520
Host Gene: RPPH1
Genomic location(hg19): chr14:20811436-20811559:-
Genomic location(hg38): chr14:20343277-20343400:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; healthy brain tissues; cerebral nodules; forming tumors
Cell lines:

NHA; A172; U251
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SDC1, ki67, MMP9
Associated microRNA: miR-627-5p, miR-663a
Biological function: Circ_RPPH1 promotes glioma cell viability, proliferation, migration, invasion and tumor growth, inhibits apoptosis, and its knockdown restrains glioma cell malignancy in vitro and in vivo.
Molecular mechanism: Circ_RPPH1 functions as a ceRNA sponge for miR-627-5p and miR-663a, thereby inducing SDC1 expression and promoting glioma malignancy.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; Transfection; MTT; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

circ_RPPH1 might be an effective therapeutic target for glioma.
Description:

circ_RPPH1 is up-regulated in glioma tissues and glioma cell lines. It promotes glioma cell proliferation, migration, invasion and in vivo tumor growth while suppressing apoptosis. Mechanistically, circ_RPPH1 acts through a miR-627-5p/miR-663a/SDC1 regulatory axis, suggesting a potential therapeutic target for glioma.
Confidence score:

0.7663
Other information
Title:

Circ_RPPH1 regulates glioma cell malignancy by binding to miR-627-5p/miR-663a to induce SDC1 expression.

Journal: Metabolic brain disease
Published: 2022
PubMed ID: 35334040
Study type:

combined biological and clinical study
Data availability: GSE146463; GSE142837; Data availability Not applicable.
Code availability: -