circRNA basic information
circBase ID: -
Name: hsa_circ_PLCD1
Synonym: circ-PLCD1
Host Gene: PLCD1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent normal tissues; tumor tissues; lung tissues
Cell lines:

NCI-H23; A549; SPC-A1; HCC827; NCI-H1299; HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: p53, PTEN, AKT, p-AKT
Associated microRNA: miR-375, miR-1179
Biological function: Circ-PLCD1 functions as a tumor suppressor in NSCLC; its overexpression inhibits cell proliferation, invasion, tumor growth and lung metastasis, and induces apoptosis.
Molecular mechanism: p53 transcriptionally activates circ-PLCD1; cytoplasmic circ-PLCD1 acts as a ceRNA sponge for miR-375 and miR-1179, elevates PTEN, and inactivates PI3K/AKT signaling.
Biological pathway or process:

PI3K/AKT (inhibits); proliferation (inhibits); invasion (inhibits); apoptosis (promotes); metastasis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; circRNA-seq; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circ-PLCD1 expression was associated with larger tumor size, later clinical stage and shorter survival time; circ-PLCD1 may be a promising prognostic biomarker for NSCLC.
Description:

circ-PLCD1 is a p53-activated tumor-suppressive circRNA that is downregulated in NSCLC tissues and cell lines. It localizes mainly in the cytoplasm and functions as a ceRNA for miR-375 and miR-1179, increasing PTEN and suppressing PI3K/AKT signaling. Higher circ-PLCD1 expression is associated with longer survival, while restoration of circ-PLCD1 inhibits NSCLC proliferation, invasion, tumor growth and metastasis and promotes apoptosis.
Confidence score:

0.887
Other information
Title:

Circular RNA circ-PLCD1 functions as a tumor suppressor in non-small cell lung cancer by inactivation of PI3K/AKT signaling pathway.

Journal: Human cell
Published: 2022
PubMed ID: 35301686
Study type:

combined biological and clinical study
Data availability: Supplementary Table 1; Supplementary Fig. 1; Supplementary Fig. 2; Supplementary Fig. 3; Supplementary Fig. 4; https:// doi. org/ 10. 1007/ s13577-022-00691-8
Code availability: -