circRNA basic information
circBase ID: hsa_circ_0001146
Name: hsa_circ_CPNE1
Synonym: -
Host Gene: CPNE1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: nucleus and cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

OS cancer tissue; adjacent normal tissues / normal bone tissues; xenograft tumor tissues
Cell lines:

MG-63; 143B
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MNAT1
Associated microRNA: miR-26a-5p
Biological function: promotes osteosarcoma cell viability, migration and invasion, and inhibits apoptosis; promotes xenograft tumor growth
Molecular mechanism: Acts as a miRNA sponge (ceRNA) for miR-26a-5p, competitively relieving miR-26a-5p-mediated repression of MNAT1 mRNA, thereby increasing MNAT1 expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

In osteosarcoma, hsa-circ-0001146 (from CPNE1) is up-regulated and localizes to both nucleus and cytoplasm. It sponges miR-26a-5p, competitively reducing miR-26a-5p binding to MNAT1 mRNA and increasing MNAT1 expression, thereby promoting proliferation/viability, migration and invasion while reducing apoptosis; knockdown also suppresses xenograft tumor growth.
Confidence score:

0.7094
Other information
Title:

The regulatory effect of has-circ-0001146/miR-26a-5p/MNAT1 network on the proliferation and invasion of osteosarcoma.

Journal: Bioscience reports
Published: 2020
PubMed ID: 32453410
Study type:

combined biological and clinical study
Data availability: GSE65071; TCGA
Code availability: -