| Expression pattern: |
UP |
| Associated gene: |
TNFAIP1, AXL, AGO2, Bax, Bcl-2, cleaved caspase-3, GRP78, CHOP, cleaved caspase-12 |
| Associated microRNA: |
miR-431-5p |
| Biological function: |
Promotes Abeta25-35-induced neuronal apoptosis and endoplasmic reticulum stress; inhibition of circ_0002945 increases cell viability and reduces apoptosis and endoplasmic reticulum stress in Abeta25-35-stimulated SK-N-SH cells and human primary neurons. |
| Molecular mechanism: |
circ_0002945 acts as a competing endogenous RNA that binds miR-431-5p and controls TNFAIP1 expression via miR-431-5p competition, thereby influencing Abeta25-35-induced apoptosis and endoplasmic reticulum stress. |
| Biological pathway or process: |
apoptosis (promotes); other pathway/process (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; MTT; Annexin V/PI Flow Cytometry; Western Blot; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_0002945 is upregulated in AD serum and may be a potential biomarker for the risk prediction and detection of AD. |
| Description: |
circ_0002945 is upregulated in AD serum and Abeta25-35-stimulated SK-N-SH cells and human primary neurons. It promotes Abeta-induced neuronal apoptosis and endoplasmic reticulum stress through a ceRNA mechanism in which circ_0002945 binds miR-431-5p and thereby increases TNFAIP1 expression. Its serum upregulation suggests potential clinical relevance for AD detection or risk prediction. |
| Confidence score: |
0.8391 |