| Expression pattern: |
DN |
| Associated gene: |
RPRD1A, beta-catenin, TCF4, SOX9 |
| Associated microRNA: |
miR-708-5p |
| Biological function: |
suppresses gastric cancer stem cell-like properties, inhibits proliferation and migration, and enhances sensitivity to oxaliplatin (OXA) |
| Molecular mechanism: |
ceRNA sponge of miR-708-5p to increase RPRD1A; RPRD1A competitively disrupts TCF4-beta-catenin transcription complex to suppress SOX9 via Wnt/beta-catenin signaling |
| Biological pathway or process: |
Wnt/beta-catenin (inhibits); stemness (inhibits); proliferation (inhibits); migration (inhibits); chemoresistance (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Survival Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RNase R Treatment; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Western Blot; Co-IP; In Vivo Animal Model; IHC (Immunohistochemistry); IF (Immunofluorescence); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
High Circ-0075305 expression is associated with better overall survival (OS), particularly in patients treated with postoperative adjuvant chemotherapy for gastric cancer. |
| Description: |
Circ-0075305 is down-regulated in gastric cancer and acts as a ceRNA that sponges miR-708-5p to increase RPRD1A. Increased RPRD1A disrupts the TCF4-beta-catenin transcription complex, inhibiting Wnt/beta-catenin signaling and SOX9-related stemness, thereby suppressing proliferation/migration and enhancing oxaliplatin sensitivity; higher Circ-0075305 predicts better OS in postoperative chemotherapy patients. |
| Confidence score: |
0.8837 |