circRNA basic information
circBase ID: hsa_circ_0003221
Name: hsa_circ_PTK2
Synonym: circPTK2
Host Gene: PTK2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005494
MONDO name: triple-negative breast carcinoma
Disease details: triple-negative breast cancer
Disease DO ID:
 0060081
Disease MeSH ID:
 D064726
Disease NCIt ID:
 C71732
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; para-cancerous normal tissues; TNBC tissues; adjacent non-cancerous tissue samples
Cell lines:

BT-549; SUM-159; MDA-MB-231; MDA-MB-453; MDA-MB-468; MCF-10A
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: NFIB, AKT/PI3K pathway, AGO2
Associated microRNA: miR-136
Biological function: promotes TNBC cell viability, proliferation, migration, invasion and EMT; promotes TNBC progression and development
Molecular mechanism: circPTK2 acts as a miR-136 sponge to enhance NFIB expression and activate the PI3K/AKT signaling pathway
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Transwell Assay; Western Blot; Nuclear-Cytoplasmic Fractionation; Survival Analysis; Bioinformatics Analysis
Clinical significance:

high circPTK2 expression is negatively related to overall survival and circPTK2/miR-136/NFIB might be an effective biomarker and therapeutic target for TNBC
Description:

circPTK2 is up-regulated in TNBC tissues and cell lines and high expression is associated with poor overall survival. Functionally, circPTK2 promotes TNBC cell viability, proliferation, migration, invasion and EMT. Mechanistically, it acts as a miR-136 sponge to up-regulate NFIB and activate PI3K/AKT signaling, suggesting the circPTK2/miR-136/NFIB axis as a potential biomarker and therapeutic target.
Confidence score:

0.8432
Other information
Title:

Circular RNA circPTK2 modulates migration and invasion via miR-136/NFIB signaling on triple-negative breast cancer cells in vitro.

Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.]
Published: 2022
PubMed ID: 35234989
Study type:

combined biological and clinical study
Data availability: Supplementary Table 1; Supplementary Methods; online supplementary material available at https://doi.org/10.1007/s00011-022-01548-4
Code availability: -