circRNA basic information
circBase ID: hsa_circ_0001721
Name: hsa_circ_CDK14
Synonym: circ-CDK14
Host Gene: CDK14
Genomic location(hg19): chr7:90355880-90356126:+
Genomic location(hg38): chr7:90726566-90726812:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; matched non-tumor tissues; xenograft tumors
Cell lines:

MG63; HOS; U2OS; Saos-2; hFOB1.19
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: E2F2, Ago2, HK-2, GLUT1
Associated microRNA: miR-198
Biological function: circ-CDK14 promotes OS cell viability, migration, invasion, glycolysis and tumor growth, and inhibits apoptosis; circ-CDK14 silencing suppresses OS progression in vitro and in vivo.
Molecular mechanism: circ-CDK14 functions as a sponge of miR-198 to regulate E2F2 expression in OS cells.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); metastasis (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model
Clinical significance:

circ-CDK14 expression was closely correlated with TNM stage and distant metastasis of OS tumors; the study suggests circ-CDK14 as a potential therapeutic target for OS.
Description:

circ-CDK14 is up-regulated in osteosarcoma tissues and cell lines. Functionally, circ-CDK14 supports OS malignant phenotypes including cell viability, migration, invasion, glycolysis and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ-CDK14 acts as a miR-198 sponge to regulate E2F2 expression, forming a circ-CDK14/miR-198/E2F2 regulatory axis with potential therapeutic relevance.
Confidence score:

0.7289
Other information
Title:

Silencing of circ-CDK14 suppresses osteosarcoma progression through the miR-198/E2F2 axis.

Journal: Experimental cell research
Published: 2022
PubMed ID: 35218724
Study type:

combined biological and clinical study
Data availability: Supplementary data to this article can be found online at https://doi. org/10.1016/j.yexcr.2022.113082.
Code availability: -