circRNA basic information
circBase ID: hsa_circ_0011232
Name: hsa_circ_PUM1
Synonym: circ_0011232
Host Gene: PUM1
Genomic location(hg19): chr1:31422973-31501711:-
Genomic location(hg38): chr1:30950126-31028864:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissues; adjacent normal tissues; xenograft tumors
Cell lines:

MHCC97-L; Huh7; MIHA
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT3
Associated microRNA: miR-503-5p
Biological function: Circ_0011232 promotes HCC cell proliferation, colony formation, migration, invasion, and tumor growth, and inhibits apoptosis.
Molecular mechanism: Circ_0011232 functions as a ceRNA by sponging miR-503-5p, thereby increasing AKT3 expression and promoting HCC progression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); mRNA stability (other)
Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis; Cohort Study
Clinical significance:

High circ_0011232 expression was associated with tumor size, TNM stage, liver cirrhosis, lymph node metastases, and shorter overall survival; it might be a prognostic biomarker and therapeutic target for HCC.
Description:

Circ_0011232 is upregulated in HCC tissues and cells and is associated with poorer overall survival and aggressive clinicopathological features. Functionally, it promotes proliferation, colony formation, migration, invasion, and xenograft tumor growth while suppressing apoptosis. Mechanistically, circ_0011232 sponges miR-503-5p to upregulate AKT3, forming a circ_0011232/miR-503-5p/AKT3 oncogenic axis.
Confidence score:

0.8283
Other information
Title:

Circ_0011232 contributes to hepatocellular carcinoma progression through miR-503-5p/AKT3 axis.

Journal: Hepatology research : the official journal of the Japan Society of Hepatology
Published: 2022
PubMed ID: 35187761
Study type:

combined biological and clinical study
Data availability: The analyzed data sets generated during the present study are available from the corresponding author on reasonable request.
Code availability: -