circRNA basic information
circBase ID: hsa_circ_0016347
Name: hsa_circ_KCNH1
Synonym: -
Host Gene: KCNH1
Genomic location(hg19): chr1:211092981-211192598:-
Genomic location(hg38): chr1:210919639-211019256:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009807
MONDO name: osteosarcoma
Disease details: osteosarcoma
Disease DO ID:
 3347
Disease MeSH ID:
 D012516
Disease NCIt ID:
 C9145
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

osteosarcoma specimens; paired non-tumour specimens; osteosarcoma tissues
Cell lines:

hFOB; U2OS; SaoS-2; HOS
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: IL6R, JAK2, STAT3, KCNH1
Associated microRNA: miR-661
Biological function: Circ_0016347 functions as an oncogene in osteosarcoma; its knockdown inhibits proliferation, migration, invasion, cell cycle progression and tumor growth, and induces apoptosis.
Molecular mechanism: Circ_0016347 targets miR-661 and regulates IL6R expression through miR-661, thereby activating the JAK2/STAT3 signalling pathway in osteosarcoma cells.
Biological pathway or process:

JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

-
Description:

Circ_0016347 is up-regulated in human osteosarcoma tissues and cells and acts as an oncogenic circRNA. It promotes proliferation, migration, invasion, cell cycle progression and tumor growth while suppressing apoptosis, at least partly through the miR-661/IL6R axis and activation of JAK2/STAT3 signaling.
Confidence score:

0.7662
Other information
Title:

Circ_0016347 modulates proliferation, migration, invasion, cell cycle, and apoptosis of osteosarcoma cells via the miR-661/IL6R axis.

Journal: Autoimmunity
Published: 2022
PubMed ID: 35166635
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -