circRNA basic information
circBase ID: hsa_circ_0020318
Name: hsa_circ_FAM53B
Synonym: CircFAM53B
Host Gene: FAM53B
Genomic location(hg19): chr10:126305648-126395456:-
Genomic location(hg38): chr10:124617079-124706887:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0021042
MONDO name: glioma
Disease details: glioma
Disease DO ID:
 -
Disease MeSH ID:
 D005910
Disease NCIt ID:
 C3059
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

glioma tissues; adjacent non-tumor tissues; cancerous and adjacent noncancerous tissues; tumor tissues
Cell lines:

U251; A172; TJ861; TJ905; LN18; HEB
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MET, c-MET, PI3K, AKT, Bax, Bcl2, E-cadherin, Vimentin, Ki67
Associated microRNA: miR-532-3p
Biological function: Promotes glioma cell proliferation, colony formation, invasion, EMT, metastasis and tumor growth, while inhibiting apoptosis.
Molecular mechanism: circFAM53B acts as a ceRNA that sponges miR-532-3p, thereby relieving miR-532-3p-mediated repression of MET/c-MET and activating the c-MET/PI3K/AKT pathway.
Biological pathway or process:

PI3K/AKT (promotes); proliferation (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Western Blot; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis
Clinical significance:

Higher circFAM53B levels were associated with poorer survival of glioma patients and the circFAM53B/miR-532-3p/c-MET/PI3K/AKT axis is proposed as a potential treatment target for glioma.
Description:

circFAM53B is up-regulated in glioma tissues and cell lines, and higher expression predicts poorer patient survival. Functionally, it promotes glioma proliferation, invasion, EMT and in vivo tumor growth while suppressing apoptosis. Mechanistically, circFAM53B sponges miR-532-3p to activate the MET/c-MET-mediated PI3K/AKT pathway.
Confidence score:

0.7789
Other information
Title:

CircFAM53B promotes the proliferation and metastasis of glioma through activating the c-MET/PI3K/AKT pathway via sponging miR-532-3p.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2022
PubMed ID: 35100091
Study type:

combined biological and clinical study
Data availability: The data sets used and analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -