circRNA basic information
circBase ID: hsa_circ_0078710
Name: hsa_circ_THBS2
Synonym: -
Host Gene: THBS2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissue; corresponding non-tumor tissue; adjacent noncancerous tissues
Cell lines:

HepG2; SMMC-7721; HuH-6; SK-Hep1; LO2; L-O20
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HDAC2, CDK2, cyclin A, cyclin D1, CDK4, p21
Associated microRNA: miR-31
Biological function: promotes HCC cell proliferation, colony formation, G1/S cell cycle progression, migration, invasion, and tumor growth in vivo
Molecular mechanism: has_circ_0078710 acts as a miR-31 sponge and upregulates miR-31 target genes HDAC2 and CDK2, leading to pro-tumor effects in HCC
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Northern Blot; RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; circRIP; Luciferase Reporter Assay; CCK8; Colony Formation Assay; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

high has_circ_0078710 expression is associated with advanced TNM stage and may serve as a promising diagnostic biomarker for HCC
Description:

has_circ_0078710 is up-regulated in HCC tissues and cell lines and is associated with advanced TNM stage. It promotes HCC proliferation, colony formation, cell-cycle progression, migration, invasion, and xenograft tumor growth. Mechanistically, it acts as a cytoplasmic miR-31 sponge to relieve repression of HDAC2 and CDK2, supporting its potential as an oncogenic biomarker in HCC.
Confidence score:

0.8354
Other information
Title:

CircRNA has_circ_0078710 acts as the sponge of microRNA-31 involved in hepatocellular carcinoma progression.

Journal: Gene
Published: 2019
PubMed ID: 30342168
Study type:

combined biological and clinical study
Data availability: Supplementary Figure 1
Code availability: -