| Expression pattern: |
UP |
| Associated gene: |
ICAM1, USP36 |
| Associated microRNA: |
miR-491-5p |
| Biological function: |
Circ_0003204 promotes ox-LDL-induced endothelial cell injuries, including inflammation, cell apoptosis, reduced cell viability, and impaired tube formation; circ_0003204 knockdown protects HUVECs against these injuries. |
| Molecular mechanism: |
Circ_0003204 acts as a ceRNA/miR-491-5p sponge to positively regulate ICAM1 expression, thereby modulating ox-LDL-induced HUVEC injuries. |
| Biological pathway or process: |
ceRNA regulation (promotes); inflammation (promotes); apoptosis (promotes); angiogenesis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; ELISA; CCK8; Annexin V/PI Flow Cytometry; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
Circ_0003204 inhibition might prevent AS development. |
| Description: |
Circ_0003204 is up-regulated in ox-LDL-treated HUVECs, an in vitro model relevant to atherosclerosis. Its knockdown protects endothelial cells by reducing inflammation and apoptosis while restoring cell viability and tube formation. Mechanistically, circ_0003204 functions as a miR-491-5p sponge to up-regulate ICAM1 through the circ_0003204/miR-491-5p/ICAM1 axis. |
| Confidence score: |
0.7406 |