| Expression pattern: |
UP |
| Associated gene: |
BPTF, BIRC6, AGO2, DHTKD1 |
| Associated microRNA: |
miR-95-5p |
| Biological function: |
Promotes LUAD cell proliferation and tumor growth, and inhibits LUAD cell apoptosis. |
| Molecular mechanism: |
ceRNA mechanism: circ_100549 sponges miR-95-5p to upregulate BPTF, and BPTF transcriptionally upregulates BIRC6. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Potential therapeutic target for LUAD treatment. |
| Description: |
circ_100549 is upregulated in LUAD cells and acts as an oncogenic circRNA. It promotes LUAD proliferation and xenograft tumor growth while inhibiting apoptosis through a ceRNA mechanism involving miR-95-5p, BPTF, and transcriptional upregulation of BIRC6. |
| Confidence score: |
0.8412 |