| Expression pattern: |
UP |
| Associated gene: |
EIF4A3, CCND1, HuR, Ago2 |
| Associated microRNA: |
miR-516b-5p |
| Biological function: |
CircCCNB1 promotes glioma cell proliferation, cell cycle progression and tumor growth; circCCNB1 knockdown suppresses glioma cell growth and arrests the cell cycle. |
| Molecular mechanism: |
EIF4A3 positively regulates circCCNB1 expression; circCCNB1 elevates CCND1 by sponging miR-516b-5p and by interacting with HuR to enhance CCND1 mRNA stability. |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes); mRNA stability (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
CircCCNB1 was overexpressed in glioma tissues and may provide a novel strategy for glioma treatment. |
| Description: |
circCCNB1 is up-regulated in glioma tissues and cell lines and functions as an oncogenic circRNA. EIF4A3 induces circCCNB1 expression, while circCCNB1 promotes glioma proliferation, cell cycle progression and xenograft tumor growth by elevating CCND1 through miR-516b-5p sponging and HuR-mediated CCND1 mRNA stabilization. |
| Confidence score: |
0.8571 |