circRNA basic information
circBase ID: hsa_circ_0007290
Name: hsa_circ_FUNDC1
Synonym: circ_0007290
Host Gene: FUNDC1
Genomic location(hg19): chrX:44383247-44386611:-
Genomic location(hg38): chrX:44524001-44527365:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
1060198
MONDO name: ischemic stroke
Disease details: ischemic stroke
Disease DO ID:
-
Disease MeSH ID:
-
Disease NCIt ID:
-
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma

Cell lines:

HCN-2

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: PDCD4
Associated microRNA: miR-496
Biological function: circ_0007290 promotes OGD-induced neuronal injury, apoptosis, LDH release, and inflammation; knockdown of circ_0007290 protects HCN-2 cells by restoring proliferation and reducing apoptosis and inflammatory cytokines.
Molecular mechanism: circ_0007290 functions as a ceRNA sponge for miR-496, relieving miR-496-mediated repression of PDCD4 and thereby regulating OGD-induced neuronal injury.
Biological pathway or process:

ceRNA regulation (other); proliferation (inhibits); apoptosis (promotes); inflammation (promotes); other pathway/process (promotes)

Detected method:
Q
M
Validation methods:

RNase R Treatment; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; ELISA; Western Blot; Bioinformatics Analysis

Clinical significance:

circ_0007290 was elevated in plasma of AIS patients and correlated with WBC, HDL-C, LDL-C, and total cholesterol, suggesting association with AIS clinical characteristics.

Description:

circ_0007290 is up-regulated in plasma from AIS patients and in OGD-treated HCN-2 cells. Its knockdown protects against OGD-induced neuronal injury by promoting cell proliferation and reducing LDH release, apoptosis, and inflammatory cytokines. Mechanistically, circ_0007290 sponges miR-496 to up-regulate PDCD4 in a circ_0007290/miR-496/PDCD4 ceRNA axis.

Confidence score:

0.7893

Other information
Title:

Knockdown of circ_0007290 alleviates oxygen-glucose deprivation-induced neuronal injury by regulating miR-496/PDCD4 axis.

Journal: Metabolic brain disease
Published: 2022
PubMed ID: 35032277
Study type:

combined biological and clinical study

Data availability: GSE133769; data sets generated during the present study are available from the corresponding author on reasonable request
Code availability: -