| Expression pattern: |
UP |
| Associated gene: |
CDK4, Ago2 |
| Associated microRNA: |
miR-338-3p |
| Biological function: |
promotes proliferation, migration, invasion and cell cycle progression of cervical cancer cells; silencing suppresses tumor growth in vivo |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-338-3p to upregulate CDK4 (Ago2-dependent binding). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; CCK8; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
circ_0000326 upregulation depended on the stage of cancer. |
| Description: |
circ_0000326 is up-regulated in cervical cancer tissues and cell lines and is associated with more advanced stage. Functionally, circ_0000326 promotes proliferation, cell-cycle progression, migration and invasion, and its knockdown suppresses xenograft tumor growth. Mechanistically, it acts as a miR-338-3p sponge in an Ago2-dependent manner to upregulate CDK4. |
| Confidence score: |
0.8429 |