| Expression pattern: |
UP |
| Associated gene: |
CTGF |
| Associated microRNA: |
miR-377-3p |
| Biological function: |
Circ-MKLN1 promotes TGF-beta2-induced PCO-related cell viability, migration, invasion and EMT in SRA01/04 cells; circ-MKLN1 knockdown inhibits these effects. |
| Molecular mechanism: |
Circ-MKLN1 acts as a miR-377-3p sponge to increase CTGF expression, thereby promoting TGF-beta2-induced PCO progression in vitro. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ-MKLN1 might be used as a molecular target for PCO therapy. |
| Description: |
Circ-MKLN1 is up-regulated in PCO lens samples and TGF-beta2-treated SRA01/04 cells. It promotes PCO-related cell viability, migration, invasion and EMT by sponging miR-377-3p and increasing CTGF expression, suggesting potential value as a therapeutic target for PCO. |
| Confidence score: |
0.7404 |