| Expression pattern: |
UP |
| Associated gene: |
NET1, Ago2, SPECC1 |
| Associated microRNA: |
miR-494-3p |
| Biological function: |
promotes ALL progression, cell cycle progression and glycolytic metabolism; inhibits apoptosis and ferroptosis |
| Molecular mechanism: |
circ_0000745 acts as a miR-494-3p sponge to positively regulate NET1 expression |
| Biological pathway or process: |
ceRNA regulation (promotes); cell cycle (promotes); glycolysis (promotes); apoptosis (inhibits); ferroptosis (inhibits); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Annexin V/PI Flow Cytometry; Cell Cycle Assay; MTT; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ_0000745/miR-494-3p/NET1 axis may provide novel potential targets for the diagnosis and treatment of ALL. |
| Description: |
circ_0000745 is up-regulated in peripheral blood samples from ALL patients and in ALL cell lines. Functionally, circ_0000745 promotes ALL cell cycle progression and glycolytic metabolism while inhibiting apoptosis and ferroptosis. Mechanistically, it acts as a miR-494-3p sponge to up-regulate NET1 expression, suggesting the circ_0000745/miR-494-3p/NET1 axis as a potential diagnostic and therapeutic target in ALL. |
| Confidence score: |
0.8111 |