| Expression pattern: |
UP |
| Associated gene: |
JAK2 |
| Associated microRNA: |
miR-375 |
| Biological function: |
hsa_circ_101280 promotes HCC cell proliferation and in vivo tumorigenesis, inhibits apoptosis, and facilitates tumorigenesis of HCC. |
| Molecular mechanism: |
hsa_circ_101280 acts as a sponge of miR-375 and upregulates JAK2, thereby regulating the miR-375/JAK2 axis in HCC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Microarray; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
hsa_circ_101280 can be used as an HCC biomarker and can be detected by qRT-PCR and in situ hybridization. |
| Description: |
hsa_circ_101280 is up-regulated in HCC tissues and cell lines and acts as an oncogenic circRNA. It promotes HCC cell proliferation and xenograft tumor growth while suppressing apoptosis, mainly by sponging miR-375 and increasing JAK2 expression. The study suggests hsa_circ_101280 may serve as a biomarker and therapeutic target for HCC. |
| Confidence score: |
0.7785 |