| Expression pattern: |
UP |
| Associated gene: |
RAC1 |
| Associated microRNA: |
miR-885-5p |
| Biological function: |
promotes PTC progression and proliferation; inhibits cell cycle arrest and apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0004458 sponges miR-885-5p to activate RAC1 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; divergent primers PCR; Sanger Sequencing; Back-Splice Junction PCR; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model |
| Clinical significance: |
hsa_circ_0004458 expression was related to tumor size, invasion, lymphatic metastasis, distal metastasis, and TNM stage; may serve as a potential therapeutic target and biomarker for PTC |
| Description: |
In papillary thyroid carcinoma, hsa_circ_0004458 is up-regulated and promotes tumor cell proliferation and in vivo tumor growth while inhibiting cell cycle arrest and apoptosis. Mechanistically, it acts as a ceRNA that sponges miR-885-5p, thereby relieving repression of RAC1 and activating RAC1 expression. |
| Confidence score: |
0.7256 |