| Expression pattern: |
UP |
| Associated gene: |
IMP3, HK2 mRNA |
| Associated microRNA: |
- |
| Biological function: |
Promotes proliferation, migration and invasion; inhibits apoptosis; promotes aerobic glycolysis and tumor growth/progression in cervical cancer. |
| Molecular mechanism: |
circCDKN2B-AS1 binds the RBP IMP3 and promotes IMP3 binding to the 3′UTR of HK2 mRNA, thereby stabilizing HK2 mRNA and enhancing HK2 expression to promote aerobic glycolysis. |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Northern Blot; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Western Blot; circRNA-seq; Clinical Sample Validation; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining |
| Clinical significance: |
Higher circCDKN2B-AS1 expression was positively correlated with poorer prognostic parameters (tumor size, FIGO stage, deep stromal invasion, lymph node metastasis). |
| Description: |
circCDKN2B-AS1 is up-regulated in cervical cancer/HSIL and acts as an oncogenic circRNA. It binds the RBP IMP3 to enhance IMP3 association with the HK2 mRNA 3′UTR, stabilizing HK2 mRNA and promoting aerobic glycolysis, proliferation, migration/invasion and tumor growth in vitro and in vivo. |
| Confidence score: |
0.8705 |