circRNA basic information
circBase ID: hsa_circ_0074903
Name: hsa_circ_SLIT3
Synonym: circ_SLIT3
Host Gene: SLIT3
Genomic location(hg19): chr5:168110970-168112932:-
Genomic location(hg38): chr5:168683965-168685927:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues; adjacent normal hepatic tissues; xenograft tumors
Cell lines:

Huh7; Hep3B; THLE-2; HEK293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: CXCR4
Associated microRNA: miR-223-3p
Biological function: promotes proliferation, migration and invasion and inhibits apoptosis in HCC cells; its down-regulation mediates celastrol anti-tumor effects and suppresses tumor growth in vivo
Molecular mechanism: circ_SLIT3 acts as a miR-223-3p sponge to regulate CXCR4 expression
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Luciferase Reporter Assay; Transfection; Colony Formation Assay; MTT; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation
Clinical significance:

-
Description:

circ_SLIT3 is up-regulated in HCC tissues/cells and promotes malignant phenotypes. Celastrol suppresses HCC progression partly by down-regulating circ_SLIT3, which functions as a ceRNA to sponge miR-223-3p and thereby increase CXCR4 expression, affecting proliferation, migration/invasion and apoptosis, including in a Hep3B xenograft model.
Confidence score:

0.7856
Other information
Title:

Anti-Tumor Effect of Celastrol on Hepatocellular Carcinoma by the circ_SLIT3/miR-223-3p/CXCR4 Axis.

Journal: Cancer management and research
Published: 2021
PubMed ID: 33574707
Study type:

combined biological and clinical study
Data availability: -
Code availability: -