| Expression pattern: |
UP |
| Associated gene: |
NOTCH1 mRNA, NOTCH1 |
| Associated microRNA: |
miR-193b-3p |
| Biological function: |
Promotes T-ALL cell proliferation and cell cycle progression, and represses T-ALL cell apoptosis. |
| Molecular mechanism: |
circ_0000745 acts as a miR-193b-3p sponge/decoy to upregulate NOTCH1 expression, thereby promoting proliferation and inhibiting apoptosis in T-ALL cells. |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
circ_0000745 expression was associated with LDH level and risk stratification in T-ALL clinical samples. |
| Description: |
hsa_circ_0000745/circ_0000745 is up-regulated in T-ALL bone marrow and T-ALL cell lines. It promotes proliferation and cell cycle progression while suppressing apoptosis by sponging miR-193b-3p, relieving repression of NOTCH1 mRNA/protein expression. Its expression is associated with clinical pathological parameters including LDH level and risk stratification. |
| Confidence score: |
0.7795 |