circRNA basic information
circBase ID: hsa_circ_0004442
Name: hsa_circ_TLK1
Synonym: circTLK1
Host Gene: TLK1
Genomic location(hg19): chr2:171884848-171902872:-
Genomic location(hg38): chr2:171028338-171046362:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005016
MONDO name: diabetic kidney disease
Disease details: Diabetic nephropathy
Disease DO ID:
 -
Disease MeSH ID:
 D003928
Disease NCIt ID:
 C84417
Disease ICD11 ID:
 615062102
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum
Cell lines:

human mesangial cells
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: AKT, NF-kappaB, p65
Associated microRNA: miR-126-5p, miR-204-5p
Biological function: CircTLK1 promotes HG-induced human mesangial cell injury, including inflammation, oxidative stress, and ECM accumulation; circTLK1 inhibition reduces these effects.
Molecular mechanism: CircTLK1 functions as a sponge for miR-126-5p and miR-204-5p to activate the AKT/NF-kappaB pathway, thereby facilitating inflammation, oxidative stress, and ECM accumulation in HG-treated human mesangial cells.
Biological pathway or process:

NF-kappaB (promotes); inflammation (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; ELISA; Western Blot
Clinical significance:

CircTLK1 was elevated in the serum of DN patients, suggesting relevance to DN pathogenesis.
Description:

In this study, circTLK1 was up-regulated in serum from DN patients and in HG-treated human mesangial cells. CircTLK1 promoted HG-induced inflammation, oxidative stress, and ECM accumulation by sponging miR-126-5p and miR-204-5p to activate the AKT/NF-kappaB pathway. Silencing circTLK1 alleviated mesangial cell injury, suggesting a mechanistic role in DN pathogenesis.
Confidence score:

0.7689
Other information
Title:

CircTLK1 Downregulation Attenuates High Glucose-Induced Human Mesangial Cell Injury by Blocking the AKT/NF-kappaB Pathway Through Sponging miR-126-5p/miR-204-5p.

Journal: Biochemical genetics
Published: 2022
PubMed ID: 34731387
Study type:

combined biological and clinical study
Data availability: -
Code availability: -