circRNA basic information
circBase ID: hsa_circ_0007874
Name: hsa_circ_MTO1
Synonym: circMTO1
Host Gene: MTO1
Genomic location(hg19): chr6:74175931-74176329:+
Genomic location(hg38): chr6:73466208-73466606:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0100430
MONDO name: fibrotic liver disease
Disease details: liver fibrosis
Disease DO ID:
-
Disease MeSH ID:
-
Disease NCIt ID:
-
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum

Cell lines:

LX-2; HEK293T; HepG2; SMMC-7721

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DN
Associated gene: Smad7, Ago2
Associated microRNA: miR-17-5p
Biological function: inhibits hepatic stellate cells activation and suppresses liver fibrosis progression; inhibits cell proliferation and cell cycle progression in TGF-beta1-treated HSCs
Molecular mechanism: circMTO1 acts as a miRNA sponge for miR-17-5p, thereby regulating Smad7 and suppressing TGF-beta1-induced HSC activation
Biological pathway or process:

ceRNA regulation (promotes); proliferation (inhibits); cell cycle (inhibits); fibrosis (inhibits)

Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Western Blot; Clinical Sample Validation; ROC Analysis; Survival Analysis

Clinical significance:

Serum circMTO1 was negatively correlated with fibrosis stages as well as HAI scores and may serve as a diagnostic biomarker for liver fibrosis in CHB patients; in HCC, high circMTO1 expression was associated with higher survival rate.

Description:

circMTO1 is down-regulated in serum of CHB patients with liver fibrosis and decreases further with increasing fibrosis/HAI scores, supporting diagnostic potential. Functionally, circMTO1 suppresses TGF-beta1-induced hepatic stellate cell activation by sponging miR-17-5p, thereby relieving repression of Smad7.

Confidence score:

0.7972

Other information
Title:

CircMTO1 inhibits liver fibrosis via regulation of miR-17-5p and Smad7.

Journal: Journal of cellular and molecular medicine
Published: 2019
PubMed ID: 31148365
Study type:

combined biological and clinical study

Data availability: All data generated or analysed during this study are included in this article.
Code availability: -