| Expression pattern: |
UP |
| Associated gene: |
KLF12, Ago2 |
| Associated microRNA: |
miR-876-3p |
| Biological function: |
Aggravates malignant phenotypes of gastric cancer cells by promoting viability, colony formation, migration, invasion, cell cycle progression and glycolysis, and inhibiting apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-876-3p to positively regulate KLF12 expression (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ-RNF111 is up-regulated in gastric cancer tissues/cells and promotes malignant behaviors (growth, migration/invasion, cell-cycle progression and glycolysis) while suppressing apoptosis. Mechanistically, it functions as a ceRNA by sponging miR-876-3p to increase KLF12 expression, and its knockdown inhibits xenograft tumor growth. |
| Confidence score: |
0.7858 |