circRNA basic information
circBase ID: -
Name: hsa_circ_BABAM1
Synonym: circRNA_102481
Host Gene: BABAM1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
3908
Disease MeSH ID:
D002289
Disease NCIt ID:
C2926
Disease ICD11 ID:
-
Disease OMIM ID:
-
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum exosomes

Cell lines:

PC9; PC9/GR; HCC827; HCC827/ER

In vivo animal model:

-

circRNA-disease information
Expression pattern:
UP
Associated gene: ROR1
Associated microRNA: miR-30a-5p
Biological function: promotes EGFR-TKIs resistance and proliferation; inhibits apoptosis in NSCLC cells
Molecular mechanism: Exosomal circRNA_102481 acts as a miR-30a-5p sponge to upregulate ROR1 expression, contributing to EGFR-TKIs resistance.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); drug resistance (promotes); ceRNA regulation (promotes)

Detected method:
Q
M
Validation methods:

Microarray; RNase R Treatment; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Annexin V/PI Flow Cytometry; Western Blot; Survival Analysis

Clinical significance:

High exosomal circRNA_102481 is associated with TNM stage, tumor differentiation status, brain metastasis, and shorter PFS and OS; may serve as a diagnostic biomarker and therapeutic target for EGFR-TKIs resistance in NSCLC.

Description:

This study reports that tumor-derived exosomal circRNA_102481 is up-regulated in EGFR-TKI-resistant NSCLC and promotes resistance by enhancing proliferation and inhibiting apoptosis. Mechanistically, circRNA_102481 functions as a ceRNA that sponges miR-30a-5p to increase ROR1 expression. High exosomal circRNA_102481 correlates with adverse clinicopathological features and shorter PFS/OS.

Confidence score:

0.814

Other information
Title:

Tumor-derived exosomal circRNA_102481 contributes to EGFR-TKIs resistance via the miR-30a-5p/ROR1 axis in non-small cell lung cancer.

Journal: Aging
Published: 2021
PubMed ID: 33952725
Study type:

combined biological and clinical study

Data availability: The datasets used/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -