| Expression pattern: |
UP |
| Associated gene: |
BCL2 |
| Associated microRNA: |
miR-432-5p |
| Biological function: |
circ_0000376 promotes OS cell viability, migration, invasion, and glycolytic metabolism, inhibits apoptosis, and attenuates the anti-tumor effects of Tan I; circ_0000376 knockdown enhances Tan I-induced suppression of xenograft tumor growth. |
| Molecular mechanism: |
circ_0000376 acts as a miR-432-5p sponge to up-regulate BCL2 expression in OS cells, forming a circ_0000376/miR-432-5p/BCL2 axis through which Tan I exerts anti-tumor activity. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
circ_0000376 was abnormally up-regulated in OS tissues and cell lines; the study suggests the circ_0000376/miR-432-5p/BCL2 axis provides novel targets for managing OS, but prognostic value was not established. |
| Description: |
circ_0000376 is up-regulated in osteosarcoma tissues and cell lines and functions as an oncogenic circRNA. Tan I suppresses OS progression partly by down-regulating circ_0000376, which otherwise sponges miR-432-5p to increase BCL2 expression and promote viability, migration, invasion, and glycolysis while inhibiting apoptosis. Silencing circ_0000376 enhances the anti-tumor effect of Tan I in a U2OS xenograft model. |
| Confidence score: |
0.7126 |