| Expression pattern: |
UP |
| Associated gene: |
ROCK1 |
| Associated microRNA: |
miR-375 |
| Biological function: |
Promotes colony formation, migration, invasion, cell cycle progression, glycolysis, and tumor growth; inhibits apoptosis in rectal cancer. |
| Molecular mechanism: |
CircTUBGCP3 acts as a cytoplasmic miRNA sponge for miR-375 and promotes malignant phenotypes by regulating the miR-375/ROCK1 axis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); cell cycle (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Potential therapeutic target for rectal cancer treatment. |
| Description: |
CircTUBGCP3 is up-regulated in rectal cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes colony formation, migration, invasion, cell cycle progression, glycolysis, and xenograft tumor growth while suppressing apoptosis, at least partly by sponging miR-375 and regulating ROCK1 expression. |
| Confidence score: |
0.7859 |