| Expression pattern: |
UP |
| Associated gene: |
STAT3, SIRT1 |
| Associated microRNA: |
- |
| Biological function: |
circPTPN22 promotes pancreatic cancer cell proliferation and tumor growth, attenuates antitumor immune cell infiltration in the tumor microenvironment, and promotes STAT3 acetylation. |
| Molecular mechanism: |
circPTPN22 binds STAT3 and promotes STAT3 acetylation by inhibiting the STAT3/SIRT1 interaction, thereby regulating proliferation and the pancreatic cancer immune microenvironment. |
| Biological pathway or process: |
proliferation (promotes); immune regulation (inhibits); JAK/STAT (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; ISH (In Situ Hybridization); FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
circPTPN22 expression was positively correlated with tumor diameter, but not with age, gender, histological grade, T/N/M stage, or clinical stage. |
| Description: |
circPTPN22 is up-regulated in pancreatic cancer tissues and cell lines. Functionally, it promotes pancreatic cancer cell proliferation and tumor growth while weakening antitumor immune infiltration. Mechanistically, circPTPN22 interacts with STAT3 and promotes STAT3 acetylation by inhibiting the STAT3/SIRT1 interaction. |
| Confidence score: |
0.8397 |