circRNA basic information
circBase ID: hsa_circ_0067835
Name: hsa_circ_IFT80
Synonym: -
Host Gene: IFT80
Genomic location(hg19): chr3:160073800-160099506:-
Genomic location(hg38): chr3:160356012-160381718:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma serum exosomes; CRC tissues; adjacent normal tissues
Cell lines:

HCT116; DLD1; SW480; RKO; HT-29; HCoEpiC; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: HOXB7, Ago2
Associated microRNA: miR-1236-3p
Biological function: promotes CRC cell proliferation and tumor growth; promotes migration and invasion; promotes EMT; inhibits apoptosis; promotes cell-cycle progression
Molecular mechanism: ceRNA mechanism: circIFT80 sponges miR-1236-3p to modulate HOXB7 expression
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); cell cycle (promotes); ceRNA regulation (promotes)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis
Clinical significance:

Increased expression of circIFT80 in CRC patients is associated with increased tumor size and advanced stage; circIFT80 might be a novel clinical molecular marker for the prognosis of CRC patients.
Description:

circIFT80 (hsa_circ_0067835), derived from IFT80, is up-regulated in CRC plasma exosomes, tumor tissues, and CRC cell lines. It mainly localizes to the cytoplasm and promotes CRC proliferation, migration, invasion and EMT while inhibiting apoptosis, by acting as a ceRNA that sponges miR-1236-3p to increase HOXB7 expression.
Confidence score:

0.8512
Other information
Title:

circIFT80 Functions as a ceRNA of miR-1236-3p to Promote Colorectal Cancer Progression.

Journal: Molecular therapy. Nucleic acids
Published: 2019
PubMed ID: 31648103
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -