| Expression pattern: |
DN |
| Associated gene: |
HDAC4, PCNA, IL-1beta, IL-6, TNF-alpha, Ago2, XPNPEP3 |
| Associated microRNA: |
miR-135b-5p |
| Biological function: |
Circ_0008360 suppresses proliferation, migration and inflammation, promotes apoptosis and caspase-3 activity, and may inhibit RA progression in RA-FLSs. |
| Molecular mechanism: |
Circ_0008360 acts as a ceRNA/miRNA sponge for miR-135b-5p, thereby upregulating HDAC4 expression and regulating RA-FLS proliferation, migration, inflammation and apoptosis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); migration (inhibits); inflammation (inhibits); apoptosis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; CCK8; Wound Healing Assay; ELISA; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
Circ_0008360 was reduced in RA synovial tissues and RA-FLSs and was proposed as a potential novel therapeutic target for RA. |
| Description: |
Circ_0008360 is downregulated in RA synovial tissues and RA-FLSs. Functionally, it suppresses RA-FLS proliferation, migration and inflammatory cytokine production while promoting apoptosis. Mechanistically, circ_0008360 sponges miR-135b-5p to upregulate HDAC4, suggesting a protective role and potential therapeutic relevance in RA. |
| Confidence score: |
0.8275 |