| Expression pattern: |
UP |
| Associated gene: |
MDM4 |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
circ-FAM120A promotes RA development by enhancing RA-FLS proliferation, migration, invasion, and inflammation, while reducing cell cycle arrest; circ-FAM120A overexpression counteracts the suppressive effects of paeoniflorin on RA-FLS progression. |
| Molecular mechanism: |
circ-FAM120A functions as a ceRNA/miRNA sponge for miR-671-5p, thereby regulating MDM4 expression; paeoniflorin ameliorates RA-FLS progression by modulating the circ-FAM120A/miR-671-5p/MDM4 axis. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); migration (promotes); invasion (promotes); inflammation (promotes); cell cycle (promotes); p53 signaling (inhibits); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; ELISA; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
The paeoniflorin/FAM120A/miR-671-5p/MDM4 axis might provide new therapeutic targets for RA. |
| Description: |
circ-FAM120A is up-regulated in RA synovial tissues and RA-FLSs and promotes RA-FLS proliferation, migration, invasion, and inflammatory cytokine production. Mechanistically, it acts as a miR-671-5p decoy to regulate MDM4, forming the circ-FAM120A/miR-671-5p/MDM4 axis through which paeoniflorin suppresses RA-FLS progression. |
| Confidence score: |
0.7416 |