circRNA basic information
circBase ID: hsa_circ_0099999
Name: hsa_circ_ZMYM2
Synonym: circ_0099999
Host Gene: ZMYM2
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0009831
MONDO name: malignant pancreatic neoplasm
Disease details: pancreatic cancer
Disease DO ID:
 1793
Disease MeSH ID:
 -
Disease NCIt ID:
 C9005
Disease ICD11 ID:
 542147910
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

pancreatic primary tumours; para-tumour normal pancreatic tissues; subcutaneous tumours
Cell lines:

Capan-1; SW1990; CFPAC-1; HPDE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FSCN1, ZMYM2, HK2, LDHA
Associated microRNA: miR-330-5p
Biological function: Promotes pancreatic cancer cell proliferation, colony formation, migration, invasion, glycolysis, and tumour growth, and inhibits apoptosis.
Molecular mechanism: circ_0099999 acts as a ceRNA that directly binds miR-330-5p and modulates FSCN1 expression, thereby regulating pancreatic carcinogenesis.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glycolysis (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

Inhibition of circ_0099999 might have therapeutic benefits in pancreatic cancer.
Description:

circ_0099999 is up-regulated in pancreatic cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, colony formation, migration, invasion, glycolysis, and xenograft tumour growth while suppressing apoptosis. Mechanistically, cytoplasmic circ_0099999 acts as a ceRNA for miR-330-5p to increase FSCN1 expression, suggesting that circ_0099999 inhibition may have therapeutic value in pancreatic cancer.
Confidence score:

0.7859
Other information
Title:

A novel circ_0099999/miR-330-5p/FSCN1 ceRNA crosstalk in pancreatic cancer.

Journal: Autoimmunity
Published: 2021
PubMed ID: 34409897
Study type:

combined biological and clinical study
Data availability: The datasets used and analysed during the current study are available from the corresponding author on reasonable request.
Code availability: -