| Expression pattern: |
UP |
| Associated gene: |
MFN2 |
| Associated microRNA: |
miR-139-5p |
| Biological function: |
Promotes IC cell proliferation, migration, EMT process, and inflammation; positively correlates with inflammatory cytokine levels in IC patients. |
| Molecular mechanism: |
circTHBS1 acts as a sponge of miR-139-5p and positively regulates MFN2, thereby facilitating IC cell proliferation, migration, EMT process, and inflammation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); EMT (promotes); inflammation (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; MTT; EdU Staining; Transwell Assay; Western Blot; ELISA; RNA Pull-Down; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
circTHBS1 expression was positively correlated with inflammatory cytokine levels in IC patients and may be a potential target for IC treatment. |
| Description: |
This study reports that circTHBS1 is up-regulated in IC tissues and primary IC cells and positively correlates with inflammatory cytokines in IC patients. Functionally, circTHBS1 promotes IC cell proliferation, migration, EMT, and inflammation by sponging miR-139-5p and up-regulating MFN2, suggesting potential therapeutic relevance in interstitial cystitis. |
| Confidence score: |
0.7289 |