| Expression pattern: |
UP |
| Associated gene: |
FZD4 |
| Associated microRNA: |
miR-654-5p |
| Biological function: |
promotes proliferation and inflammation, inhibits apoptosis in TNFalpha-induced HFLS-RA cells, and may accelerate RA progression |
| Molecular mechanism: |
circ_0003972 acts as a miR-654-5p sponge and positively regulates FZD4 expression through the miR-654-5p/FZD4 axis |
| Biological pathway or process: |
proliferation (promotes); inflammation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; CCK8; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation) |
| Clinical significance: |
circ_0003972 might be a potential therapeutic target for RA |
| Description: |
circ_0003972 is up-regulated in synovial tissues from RA patients and in HFLS-RA cells. Functionally, it promotes proliferation, cell-cycle progression and inflammatory cytokine production while suppressing apoptosis in TNFalpha-induced HFLS-RA cells. Mechanistically, circ_0003972 sponges miR-654-5p to positively regulate FZD4, suggesting a potential therapeutic role in RA. |
| Confidence score: |
0.7799 |