| Expression pattern: |
UP |
| Associated gene: |
MAPK1 |
| Associated microRNA: |
miR-16 |
| Biological function: |
circZFR acts as an oncogenic circRNA in thyroid cancer; circZFR knockdown inhibits TC cell viability and invasion and induces apoptosis, thereby retarding malignant properties and TC progression. |
| Molecular mechanism: |
circZFR functions as a ceRNA by sponging miR-16, thereby relieving miR-16-mediated repression of MAPK1 and positively regulating MAPK1 expression in TC cells. |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RNA Pull-Down; Western Blot |
| Clinical significance: |
The circZFR/miR-16/MAPK1 axis may serve as potential therapeutic targets for TC. |
| Description: |
circZFR is up-regulated in thyroid cancer tissues and cells and acts as an oncogenic circRNA. Silencing circZFR suppresses TC cell viability and invasion and promotes apoptosis. Mechanistically, circZFR sponges miR-16 to positively regulate MAPK1 expression, forming a circZFR/miR-16/MAPK1 axis that may represent a therapeutic target. |
| Confidence score: |
0.657 |